Description_x000D_ Application_x000D_ Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies ._x000D_ Biochem/physiol Actions_x000D_ Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group._x000D_ Unit Definition_x000D_ One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA._x000D_ Physical form_x000D_ Suspension in 2.8 M (NH4)2SO4 solution_x000D_ Analysis Note_x000D_ Protein determined by E1%/280