Description_x000D_ General description_x000D_ The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency._x000D_ These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications._x000D_ They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols._x000D_ They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively._x000D_ The cells have a transformation efficiency of >1 x 109 cfu/μg_x000D_ The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg._x000D_ Genotype_x000D_ F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA_x000D_ Application_x000D_ Suitable for bacterial transformations to recover high quality plasmid DNA_x000D_ Features and Benefits_x000D_ • share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols._x000D_ • ensures recovery of stable and high quality plasmid DNA._x000D_ • high transformation efficiency_x000D_ • provide the performance researchers need with ease of use._x000D_ • provide solutions for a wide range of applications at economical prices._x000D_ • Blue - white screening_x000D_ Components_x000D_ • SIG10 chemically competent cells_x000D_ • pUC 19 transformation control DNA at 10 pg/μL_x000D_ • recovery medium for cloning_x000D_ Principle_x000D_ The SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements._x000D_ This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation._x000D_ They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation._x000D_ Legal Information_x000D_ DH10B is a trademark of Life Technologies_x000D_ DH5α is a trademark of Invitrogen Corp.