Description_x000D_ General description_x000D_ Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate carbonic acid reaction is 6.4 The pKa for bicarbonate carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications._x000D_ Application_x000D_ Sodium bicarbonate has been used:_x000D_ • as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)_x000D_ • as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells_x000D_ • as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells_x000D_ • as a component of medium used for cell culture of human embryonic kidney cells_x000D_ • as a component of Beltsville thawing solution used for the dilution of semen_x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 5 kg in poly drum_x000D_ 500 g in poly bottle