Description_x000D_ General description_x000D_ Sphingosine 1-phosphate-induced release of TIMP-2 from vascular smooth muscle cells inhibits angiogenesis._x000D_ Application_x000D_ Sphingosine kinase 1 has been used in a study to assess the modulation of cellular S1P levels. Sphingosine kinase 1 has also been used in a study to investigate how FTY720 normalizes hyperglycemia._x000D_ Biochem/physiol Actions_x000D_ Converts sphingosine to sphingosine-1-phosphate. SPHK1 shares two large conserved regions with SPHK2. While SPHK1 is found only in the cytosol, SPHK2 is localized in both the cytosol and nuclei._x000D_ Unit Definition_x000D_ One unit will produce 1.0 pmole of sphingosine-1-phosphate from ATP and D-erythro-sphingosine per minute at pH 7.4, 37 °C._x000D_ Physical form_x000D_ Supplied in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween 20, 50% glycerol, 3 mM DTT.