Description General description A cell-permeable and selective inhibitor of NAD+-dependent deacetylase activity of Sir2 protein (IC50 = 60 µM). Creates a phenocopy of the sir2 deletion mutant in S. cerevisiae and sensitizes mammalian cells to a variety of DNA-damaging agents by abrogating Sir2p activity on p53. Reported to act by either altering or blocking access to the acetylated histone-binding pocket. A cell-permeable lactone derived from β-naphthol that acts as a selective inhibitor of NAD+-dependent histone deacetylase activity of Sir2 protein (IC50 = 60 µM). It creates a conditional phenocopy of a sir2 deletion mutant in S. cerevisiae and sensitizes mammalian cells to a variety of DNA-damaging agents by abrogating Sir2p activity on p53. Acts by either altering or blocking access to the acetylated histone binding pocket. Packaging 5 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: yes Primary Target NAD+-dependent histone deacetylase activity of Sir2 protein Product does not compete with ATP. Reversible: no Target IC50: 60 µM against NAD+-dependent histone deacetylase activity of Sir2 protein Packaging Packaged under inert gas Warning Toxicity: Standard Handling (A) Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Other Notes Bedalov, A., et al. 2001. Proc. Natl. Acad. Sci. USA98, 15113.