Description_x000D_ General description_x000D_ This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures._x000D_ Application_x000D_ GuI`Bert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells._x000D_ Linkage_x000D_ Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information._x000D_ Legal Information_x000D_ AQmedia is a trademark of Sigma-Aldrich Co. LLC