Description_x000D_ General description_x000D_ Chemical structure: aminoglycoside_x000D_ Packaging_x000D_ 25, 100 g in poly bottle_x000D_ Application_x000D_ Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50μg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L._x000D_ Biochem/physiol Actions_x000D_ Mode of Action: Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation._x000D_ Mode of Resistance: A mutation in rpsL, a gene for S12 ribosomal protein, prevents binding of streptomycin to the ribosome. An aminoglycoside phosphotransferase also inactivates streptomycin._x000D_ Antimicrobial spectrum: Streptomycin acts against gram-negative and gram-positive bacteria._x000D_ Caution_x000D_ Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods. At 37°C, solutions are stable for three days._x000D_ Preparation Note_x000D_ Stock solutions should be prepared directly in the vial with sterile water at concentrations between 20-25 mg/mL._x000D_ Other Notes_x000D_ Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place