Description_x000D_ Amino Acid Sequence_x000D_ Ala-Gly-Arg-pNA_x000D_ Application_x000D_ Thrombin generation chromogenic substrate can be used for chromogenic assay developed through aptamer affinity capture and a subsequent enzyme reaction for detecting the thrombin in dilute human serum._x000D_ Biochem/physiol Actions_x000D_ Thrombin generation chromogenic substrate also known as beta-Ala-Gly-Arg para-nitroanilide, is proteolytically cleaved by human thrombin and produces beta-Ala-Gly-Arg and p-nitroanilide. The release of p-nitroanilide is quatified for assesing the anti-thrombin activity.