Description_x000D_ General description_x000D_ Trichostatin A is a compound of primary hydroxamic acid._x000D_ Application_x000D_ Trichostatin A, ready-made solution has been used:_x000D_ • as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing_x000D_ • to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)_x000D_ • to treat cells for the HDAC inhibition experiments_x000D_ Biochem/physiol Actions_x000D_ Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.