Description_x000D_ General description_x000D_ TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water)._x000D_ Application_x000D_ Ready for use in gel electrophoresis after dilution to working concentrations._x000D_ Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE)._x000D_ TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids._x000D_ Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution._x000D_ Packaging_x000D_ The 4L, 10L and 20L sizes are supplied in dispenser with a spigot._x000D_ Preparation Note_x000D_ Prepared with 18 megohm water_x000D_ Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134)._x000D_ Other Notes_x000D_ TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.