Description_x000D_ General description_x000D_ Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection._x000D_ Application_x000D_ Triton™ X-100 has been used:_x000D_ • In immunohistochemistry for staining the Flat-mount retinas_x000D_ • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay_x000D_ • To permeabilise cells during Immunofluorescent microscopic studies_x000D_ • As a positive control in LDH assay to determine the cell membrane integrity_x000D_ • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure_x000D_ • For the preparation of outer membrane protein exctract_x000D_ • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells_x000D_ • In the treatment of tissue sections for Immunofluorescence labeling_x000D_ Packaging_x000D_ 50, 100, 250 mL in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions._x000D_ Legal Information_x000D_ Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow