Description General description Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection. Application Triton™ X-100 has been used: • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling • In the blocking solution during immunocytochemistry procedure • During deglycosylation procedure • For membrane solubilization • For cell permeabilization • In decellularization of porcine heart valve tissue Packaging 1 L in poly bottle 100, 500 mL in poly bottle Biochem/physiol Actions Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. Legal Information Span is a registered trademark of Croda International PLC TWEEN is a registered trademark of Croda International PLC Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow