Description_x000D_ General description_x000D_ The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol._x000D_ Application_x000D_ Trypan blue has been used:_x000D_ • in cell viability assay_x000D_ • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution._x000D_ • to stain plant tissue in order to to visualize cell death_x000D_ Packaging_x000D_ 5, 25, 100 g in glass bottle_x000D_ Biochem/physiol Actions_x000D_ The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.