Description_x000D_ General description_x000D_ Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties._x000D_ Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition._x000D_ Application_x000D_ Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies._x000D_ Packaging_x000D_ 10 mg in glass bottle_x000D_ 25 mg in poly bottle_x000D_ 50, 100, 500 mg in glass bottle_x000D_ Unit Definition_x000D_ One trypsin unit = ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path)._x000D_ Physical form_x000D_ Lyophilized powder containing phosphate buffer salts, pH 7.6_x000D_ Analysis Note_x000D_ 1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein._x000D_ Other Notes_x000D_ View more information on Trypsin Inhibitor.