Description Application Tryptone-yeast extract-glucose medium may be used in response surface methodology in order to find optimimum conditions for the growth of Lactobacillus casei YIT 9018. It was also used in the media used in the growth experiments with Escherichia coli MG165, conducted in order to monitor the bacteria for long term which underwent programmed population control, in a microchemostat, in an approach to prevent biofilm formation. Tryptone glucose extract agar (TGEA) along with gypsum board may also be used to cultivate Streptomyces albidoflavui in order to produce volatile metabolites. Tryptone may also be used in STGG (skim milk, tryptone, glucose, glycerol) medium at -80°C, which is used to preserve Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates for atleast 3 years. Packaging 1 kg in poly bottle 250 g in poly bottle Preparation Note Pancreatic digest of casein.