Description_x000D_ General description_x000D_ Ubiquitin-conjugating enzyme E2 (UBE2E2) is a protein that in humans is encoded by the UBE2E2 gene. Amino acid sequence within the UBC domain of UBE2E2 shares over 90% identity with human UbcH6, mouse UbcM2, and Drosophila UbcD2, whereas the N-terminal region shows little amino acid sequence similarity with known proteins. The UBE2E2 protein forms a thioester bond with ubiquitin in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase._x000D_ Physical form_x000D_ 75 μL of a 40 μM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol._x000D_ Preparation Note_x000D_ Centrifuge the vial prior to opening.