Description_x000D_ General description_x000D_ Subunit molecular weight: ~90,770_x000D_ Composed of six subunits with total molecular weight: ~544,620_x000D_ Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms._x000D_ Application_x000D_ Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis._x000D_ Packaging_x000D_ 20000, 100000, 500000, 1000000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline._x000D_ Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide._x000D_ Unit Definition_x000D_ One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)