Description Specificity Recognizes VAMP-2 (Vesicle-Associated Membrane Protein 2, Synaptobrevin 2). The epitope does not share homology with any other known proteins. SPECIES REACTIVITIES: It is expected that the antibody will also react with human, Macaca multata and bovine due to sequence homology (25/26). Other species have not been tested. Immunogen GST fusion protein corresponding to a cytoplasmic, N-terminal part of rat or mouse VAMP-2 (Accession numbers Q64357, NP 036795). Application Anti-VAMP-2 Antibody detects level of VAMP-2 & has been published & validated for use in WB. Research Category Neuroscience Research Sub Category Synapse & Synaptic Biology Western blot: 1:1,000 using ECL on rat brain membranes. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. Physical form Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min) Storage and Stability Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.