Description_x000D_ Application_x000D_ Withaferin A was used to treat HEK293 cells to study its effect on cystic fibrosis inflammation._x000D_ Biochem/physiol Actions_x000D_ Withaferin A is a steroidal lactone that is isolated from the plant Withania somnifera. It has potent anti-inflammatory properties as it inhibits the activation of NF-κ B signaling pathway. The anti-tumor activity of Withaferin A is due to its ability to alter cytoskeletal architecture by binding annexin II and disrupting F actin cross-links. Withaferin A also inhibits angiogenesis by binding to vimentin and F-actin._x000D_ Steroidal lactone that exhibits cytotoxicity towards tumor cells. Protective effects attributed to anti-lipid peroxidative, antioxidant and detoxifying functionality._x000D_ Preparation Note_x000D_ Withaferin A yields a clear, colorless solution in methanol at 1 mg/ml.